Prescreening Questions to Ask Environmental DNA (eDNA) Monitoring Technician

Tell us about your experience with eDNA sample collection and processing.

Experience is everything in the world of eDNA. Ask candidates to explain their hands-on experience with collecting and processing eDNA samples. Have they worked in diverse environments? What specific protocols do they follow? A well-rounded answer will reveal a candidate’s depth of understanding and capability in real-world situations.

What methods do you use to prevent contamination in eDNA samples?

Contamination is the arch-nemesis of reliable eDNA data. It's crucial to know the techniques a candidate employs to avoid this. Do they use sterile equipment? Are they meticulous about cross-contamination protocols? Their response will provide insights into their attention to detail and adherence to best practices.

How do you ensure the quality and integrity of eDNA samples in the field and lab?

Maintaining sample quality from collection to analysis is a tricky dance. Ask about their QA/QC measures. How do they handle samples in the field? What protocols are in place in the lab to ensure integrity? Their answer will tell you a lot about their methodological rigor and reliability.

Can you describe your familiarity with various eDNA extraction techniques?

The extraction technique can make or break the quality of eDNA data. Are they well-versed in methods like filtration and precipitation? Do they have experience with commercial kits? This question aims to explore the breadth of their technical skills in eDNA extraction.

What software and tools are you proficient in for analyzing eDNA data?

Data isn't much without proper analysis. Are they proficient in tools like QIIME, Mothur, or R? Maybe they have coding skills for custom scripts? Their software toolkit is crucial for interpreting eDNA results accurately.

Describe a challenging project you worked on related to eDNA monitoring and how you overcame those challenges.

Challenges are learning experiences in disguise. Ask for specifics about projects they found particularly challenging and how they navigated those difficulties. This will provide insights into their problem-solving skills and resilience.

How do you stay updated with the latest advancements and protocols in eDNA research and monitoring?

The field of eDNA is constantly evolving. It’s important to know how candidates keep themselves updated. Do they read scientific journals? Attend workshops and conferences? Their commitment to continuous learning can be a strong indicator of their passion and dedication.

Explain your experience with PCR, qPCR, or other molecular techniques used in eDNA analysis.

Molecular techniques are the bread and butter of eDNA analysis. Ask them about their hands-on experience with PCR and qPCR. Can they troubleshoot these processes? Are they familiar with other molecular techniques like metabarcoding? This question digs deep into their scientific expertise.

How do you manage and organize large datasets generated from eDNA studies?

Handling large datasets is like organizing a vast library. Inquire about their data management practices. Do they use specific software for data organization? How do they ensure data integrity? This will reveal their organizational skills and attention to detail.

Can you discuss your experience with bioinformatics and the interpretation of eDNA sequencing data?

Bioinformatics is the secret sauce in making sense of eDNA data. How experienced are they with aligning sequences, filtering data, or identifying taxa? Their ability to interpret data accurately will determine the reliability of their research findings.

Have you ever had to train others in eDNA monitoring techniques? Describe the experience.

Training is a testament to one’s mastery of a subject. Have they mentored interns or trained colleagues? How effective were they in conveying complex techniques? This will provide insight into their communication skills and leadership potential.

What protocols do you follow for the storage and transportation of eDNA samples?

Proper storage and transportation are crucial to maintaining sample integrity. What protocols do they follow to ensure samples remain uncontaminated and viable? Their attention to these details will reveal how seriously they take their work.

How do you verify the accuracy and reliability of your eDNA monitoring results?

Accuracy is the hallmark of credible eDNA work. Ask about their validation protocols. Do they use positive and negative controls? How do they confirm the timeliness and reliability of their results? This question will illustrate their commitment to scientific rigor.

Can you talk about any specific ecosystems or species you have focused on in your eDNA work?

Specialization often enhances expertise. Have they focused on marine, freshwater, or terrestrial ecosystems? Have they studied specific endangered species? Their experience in different ecosystems can add valuable context to their overall expertise.

Discuss any collaborative projects you’ve been part of in the field of eDNA monitoring.

Science is often a team sport. Collaboration brings new perspectives and skills. Ask them about their team projects. How did they contribute? What was the project's outcome? This will show their ability to work well with others.

What are some ethical considerations you take into account when conducting eDNA studies?

Ethics ensure that scientific research benefits all while minimizing harm. Ask about their considerations for environmental impacts and data privacy. Their response will provide insight into their ethical grounding and responsibility.

How do you handle unexpected results or discrepancies in your eDNA data?

Unexpected results are all part of the scientific journey. Ask how they deal with discrepancies and surprises in their data. Do they double-check their work? Consult with peers? This will highlight their problem-solving and analytical skills.

What field equipment are you most comfortable with when collecting eDNA samples?

From filtration pumps to sampling kits, equipment familiarity is crucial. Ask them about their preferred tools and technologies for fieldwork. Their comfort and experience with specific equipment can make a significant difference in data quality.

Discuss your experience with scaling up eDNA monitoring projects from pilot studies to full implementations.

Pilot studies are like training wheels for larger projects. How have they handled scaling up? What strategies did they use to ensure consistency and reliability? Their experience with full-scale implementations can indicate their ability to manage large projects.

How would you approach designing an eDNA monitoring program for a new study site?

Designing a new monitoring program requires strategic planning. Ask them about their approach to site selection, sample collection, and data analysis. Their methodology will provide a window into their thought process and expertise in project planning.