Review the evidence signals before interviewing. Then use the anchored descriptions—not instinct alone—to choose the score that best matches each answer.
01
Evaluation factor
Technique and experimental design
35% weight
Probe hands-on eDNA workflow depth: Sterivex or filter-based water sampling, extraction kits (DNeasy PowerWater), qPCR and ddPCR assay validation, metabarcoding primers such as MiFish or 12S.
Evidence to listen for
Runs the assays and instruments themselves rather than describing what a team does
Designs experiments with controls, replicates, and a stated hypothesis
Knows what each technique can and cannot resolve
Understands the science, not only the protocol
Five-point scoring guide
1
Poor
Protocol follower with no experimental design; cannot justify controls.
2
Needs Improvement
Runs standard assays; designs experiments poorly or not at all.
3
Satisfactory
Competent at the bench with sound routine design.
4
Very Good
Designs rigorous experiments and understands the limits of each technique.
5
Excellent
Names specific filtration volumes, extraction chemistries and assay limits of detection, and explains why each was chosen for the target taxa.
02
Evaluation factor
Results that went somewhere
25% weight
Ask which surveys their data actually informed: invasive species early detection, protected species presence for consenting, agency reports to EPA, Environment Agency or state wildlife bodies.
Evidence to listen for
Names projects where their results changed a decision, a process, or a product
States their own contribution rather than the group's
Has taken something from bench to a larger scale, a filing, or a publication
Knows what happened to the work after they handed it over
Five-point scoring guide
1
Poor
No results that went anywhere; work is entirely exploratory.
2
Needs Improvement
Contributed to projects but cannot say what their data changed.
3
Satisfactory
Real contributions; outcomes described loosely.
4
Very Good
Names results that changed a decision, with clear personal scope.
5
Excellent
Cites named catchments or projects where eDNA detections triggered management action, with sample counts and detection rates reported.
03
Evaluation factor
Troubleshooting and reproducibility
25% weight
Test handling of contamination and inhibition: field and extraction blanks turning positive, PCR inhibitors in turbid water, false negatives, replicate disagreement, occupancy modelling of detection probability.
Evidence to listen for
Treats a failed run as information rather than bad luck
Isolates reagent, instrument, operator, and biological causes systematically
Knows why a result failed to reproduce and can say when their own data was wrong
Keeps records good enough to diagnose from months later
Five-point scoring guide
1
Poor
Repeats failed runs unchanged; no diagnostic thinking.
2
Needs Improvement
Troubleshoots by substitution; cannot explain a reproducibility failure.
3
Satisfactory
Solid troubleshooting on familiar assays.
4
Very Good
Systematic isolation of causes, and honest about their own irreproducible results.
5
Excellent
Describes a specific contamination or inhibition incident, the diagnostic steps taken, and the controls added to prevent recurrence.
04
Evaluation factor
Documentation and collaboration
15% weight
Check chain of custody and data practice: field metadata sheets, cold chain logs, MIQE-style reporting, bioinformatics pipelines (OBITools, DADA2), sequence submission and handover to ecologists.
Evidence to listen for
Keeps records to the standard the setting requires, whether that is GLP, GMP, or a defensible notebook
Writes up so someone else can repeat the work
Works with process, quality, or clinical colleagues rather than in a bench silo
Explains a result to a non-specialist without overclaiming
Five-point scoring guide
1
Poor
Records would not survive audit; work is not repeatable from them.
2
Needs Improvement
Documentation is thin; write-ups need heavy editing.
3
Satisfactory
Adequate records and write-ups; collaboration is limited.
4
Very Good
Audit-standard records and clear communication across functions.
5
Excellent
Keeps auditable sample metadata and versioned pipeline scripts, and hands results to non-molecular ecologists with caveats stated plainly.
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